Quantitative characterization of quantum dot-labeled lambda phage for Escherichia coli detection
✍ Scribed by Peter B. Yim; Matthew L. Clarke; Michael McKinstry; Silvia H. De Paoli Lacerda; Leonard F. Pease III; Marina A. Dobrovolskaia; HyeongGon Kang; Timothy D. Read; Shanmuga Sozhamannan; Jeeseong Hwang
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 644 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
We characterize CdSe/ZnS quantum dot (QD) binding to genetically modified bacteriophage as a model for bacterial detection. Interactions among QDs, lambda (λ) phage, and Escherichia coli are examined by several cross‐validated methods. Flow and image‐based cytometry clarify fluorescent labeling of bacteria, with image‐based cytometry additionally reporting the number of decorated phage bound to cells. Transmission electron microscopy, image‐based cytometry, and electrospray differential mobility analysis allow quantization of QDs attached to each phage (4–17 QDs) and show that λ phage used in this study exhibits enhanced QD binding to the capsid by nearly a factor of four compared to bacteriophage T7. Additionally, the characterization methodology presented can be applied to the quantitative characterization of other fluorescent nanocrystal‐biological conjugates. Biotechnol. Bioeng. 2009;104: 1059–1067. Published 2009 Wiley Periodicals, Inc.