Quantitative characterization of crude synaptosomal fraction (P-2) components by flow cytometry
✍ Scribed by Karen H. Gylys; Jeffrey A. Fein; Gregory M. Cole
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 195 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Flow cytometry, which definitively identifies each particle as positive or negative with respect to fluorescent markers, is used to characterize the P-2 fraction (crude synaptosomal fraction) with respect to primary components, size, and intactness. Particle size ranged from a few tenths of a m to greater than 4.5 m. The viable dye calcein AM labeled 90% of the preparation, indicating that the majority of particles were intact and esterasepositive. 66% of the P-2 fraction is neuronal in origin, as demonstrated by labeling with an antibody directed against SNAP-2. An antibody directed against glial fibrillary acidic protein (GFAP) labeled 35% of the particles in this preparation. The mitochondrial dye nonyl acridine orange (NAO) stained 74% of particles, indicating intraand extrasynaptosomal mitochondria. Gating analysis reveals that SNAP-25 is enriched in the larger particles. These results suggest that flow cytometry may be used to take advantage of the increased viability, yield, and convenience of the P-2 fraction for studies of nerve terminal function.