Quantitative assay of deoxyribonuclease activity after isoelectric focusing in polyacrylamide gels: pH control and effects of enzyme diffusion

Chromogenic substrate autography: A meth

Chromogenic substrate autography: A method for detection, characterization, and quantitative measurement of serine proteases after sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing in polyacrylamide gels

✍ Oswald F. Wagner; Ingrid Bergmann; Bernd R. Binder 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 443 KB

A chromogenic substrate autography is described which allows characterization and quantification of serine proteases in crude systems after sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing. Separation of samples containing proteases by either method is followed by an