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Quantitative aspects of the development of a hydrophobic binding site on calmodulin by calcium binding

✍ Scribed by Robert F. Steiner

Publisher: Wiley (John Wiley & Sons)
Year: 1984
Tongue: English
Weight: 614 KB
Volume: 23
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360230612

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✦ Synopsis

The interactive binding by calmodulin of Ca2+ and 1-anilinonaphthalene-8-sulfonate (1,8-ANS) has been examined. In the presence of saturating levels of Ca2+, calmodulin develops one moderately strong binding site for 1,8-ANS, plus one or more weaker sites. The binding of 1,8-ANS by unliganded, or singly liganded, calmodulin is slight; the development of a strong binding site, as well as the characteristic fluorescence enhancement and CD spectrum, requires the binding of two Ca2+ ions. Little further change occurs on binding additional Ca2+ ions.

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