Quantitative and Qualitative Microscopy

✍ Scribed by P. Michael Conn (Eds.)

• Publisher: Academic Press
• Year: 1990
• Tongue: English
• Leaves: 492
• Series: Methods in Neurosciences 3
• Category: Library

✦ Synopsis

Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques.

A convenient bench-top formatMethods presented for easy adaptation to new systemsComprehensive protocols included forAutoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniquesStatistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arborsTracing neuronal connections: tracer, toxin, and dye methodsStaining techniques: fluoro-gold, SITS, and silver impregnationFreezing techniques: freeze fracture and cryoprotectionCombined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques

✦ Table of Contents

Content:
Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 3
Pages ix-xi

Preface
Page xiii
P. MICHAEL CONN

Methods in Neurosciences
Page xv

1 - Autoradiographic and Microchemical Methods for Quantitation of Steroid Receptors
Pages 3-35
N.J. MacLusky, T.J. Brown, E. Jones, C. Leranth, R.B. Hochberg

2 - High-Resolution Autoradiographic Mapping of Drug and Hormone Receptors
Pages 35-50
Walter E. Stumpf, Gary E. Duncan

3 - High-Resolution Autoradiographic Imaging of Brain Activity Patterns with Radiolabeled 2-Deoxyglucose and Glucose
Pages 50-64
Gary E. Duncan, Walter E. Stumpf

4 - Double- and Single-Label Quantitative Autoradiography for Cerebral Physiology
Pages 64-89
Stephen C. Jones

5 - Combination of Tritiated Thymidine Autoradiography and Neuropeptide Immunocytochemistry to Determine Birthdates and Migration Routes of Luteinizing Hormone-Releasing Hormone Neurons
Pages 90-106
Marlene Schwanzel-Fukuda, Donald W. Pfaff

6 - Techniques and Technology for Dynamic Video Imaging of Cellular Fluorescence
Pages 109-135
W.T. Mason, J. Hoyland, S.R. Rawlings, G.T. Relf

7 - Three-Dimensional Computer Reconstruction of Perforated Synapses
Pages 136-154
R.K.S. Calverley, D.G. Jones

8 - Determination of Numerical Density of Perforated and Nonperforated Synapses
Pages 155-172
R.K.S. Calverley, D.G. Jones

9 - Efficient and Unbiased Sampling of Nerve Fibers for Estimating Fiber Number and Size
Pages 172-187
Terry M. Mayhew

10 - Methods for Analyzing Neuronal Connections in Mammals
Pages 188-207
Vivien A. Casagrande, James B. Hutchins

11 - Image Analytic Techniques for Quantification of Immunohistochemical Staining in the Nervous System
Pages 208-229
Arnold J. Smolen

12 - Methods for Visualizing and Analyzing Individual Axon Arbors
Pages 230-244
Edward A. Lachica, Vivien A. Casagrande

13 - Phaseolus vulgaris Leucoagglutinin Anterograde Axonal Transport Technique
Pages 247-260
P.E. Sawchenko, E.T. Cunningham Jr., M.T. Mortrud, S.W. Pfeiffer, C.R. Gerfen

14 - Retrograde Axoplasmic Transport of Neurotoxins
Pages 261-275
Teiji Yamamoto

15 - Tracing Neuronal Connections in the Periphery: Renal Nerves
Pages 275-290
J. Michael Wyss, Wanida Sripairojthikoon

16 - Small Injections of Axonally Transported Fluorescent Tracers
Pages 291-314
George F. Alheid

17 - Fluoro-Gold and 4-Acetamido-4′-isothiocyanostilbene-2,2′-disulfonic Acid: Use of Substituted Stilbenes in Neuroanatomical Studies
Pages 317-330
Larry Schmued

18 - Silver Impregnation Method for Frozen Sections of Human Nervous Tissue Using Ammoniacal Silver–Dichromate Solution
Pages 330-334
Yoshiaki Ogawa

19 - Silver Impregnation Method for Neurons Using Synthetic Surfactants: A Contribution to Golgi Method
Pages 335-339
Yoshiaki Ogawa

20 - Use of Freeze-Fracture in Neurobiology
Pages 343-360
Joel A. Black

21 - Cryoprotection and Freezing Methods to Control Ice Crystal Artifact in Frozen Sections of Fixed and Unfixed Brain Tissue
Pages 360-385
Douglas L. Rosene, Kenneth J. Rhodes

22 - Double-Label [³H]2-Deoxyglucose and [¹⁴C]2-Deoxyglucose Method for Mapping Brain Activity Underlying Two Experimental Conditions in the Same Animal
Pages 389-412
Harriet R. Friedman, Charles J. Bruce, Patricia S. Goldman-Rakic

23 - Application of Incident Light Polarization Microscopy
Pages 413-424
Alfons B.A. Kroese, Sietse M. van Netten

24 - Light Microscopic Localization of Drug and Neurotransmitter Receptors in the Brain
Pages 425-440
Donald R. Gehlert, Henry I. Yamamura

25 - Light and Electron Microscopic Investigation of Somatostatin-Containing Neurons in the Central Nervous System
Pages 440-456
Kiminao Mizukawa

26 - Reduced Nicotinamide Adenine Dinucleotide Phosphate–Diaphorase Histochemistry: Light and Electron Microscopic Investigations
Pages 457-472
Kiminao Mizukawa

Addendum to Article [6]
Page 475
W.T. Mason, J. Hoyland, S.R. Rawlings, G.T. Relf

Index
Pages 477-494