Quantitative analysis of water-soluble vitamins in multicomponent pharmaceutical forms : Determination of tablets and granules

✍ Scribed by Tatjana Bićan-Fišter; Vojislava Dražin

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 302 KB
• Volume: 77
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92206-4

✦ Synopsis

A method has been developed for the individual determination of watersoluble vitamins in compound pharmaceutical forms after thin-layer chromatographic separation . Procedures for tablets and granules containing thiamine hydrochloride, riboflavine, pyridoxine hydrochloride, nicotinamide and fi-aminobenzoic acid are proposed. The quantitative separation of vitamins was achieved on silica gel plates, using the solvent mixture glacial acetic acid-acetone-methanol-benzene as the mobile phase, Thiamine hydrochloride, pyridoxine hydrochloride and nicotinamide were determined after extraction from the sorbent by UV spectrophotometry, while riboflavine was determined by fluorimetry and p-aminobenzoic acid by calorimetry after diazotation . Satisfactory standard deviations were obtained for all the vitamins examined .