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Quantitative analysis of the mono- and sesquiterpenoids of douglas-fir sapwood by solvent extraction and gas chromatography with mass selective detection

✍ Scribed by Kimball, Bruce A. ;Craver, Rori K. ;Johnston, John J. ;Nolte, Dale L.

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 418 KB
Volume: 18
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240180404

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✦ Synopsis

In order to investigate the relationship between terpenoid content and black bear foraging preference, an analytical method was required to quantify mono-and sesquiterpenoids present in Douglas-fir sapwood. Sapwood samples were scraped from trees, immediately frozen in liquid nitrogen, and then homogenized. A simple extraction requiring no clean-up step was performed with ethyl acetate. Extracts were analyzed by gas chromatography with mass selekve detection versus external standards. The recoveries of 22 terpenoids from fortified controls were approximately 90% with good precision (relative standard deviations of approximately 10%).

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