Quantitative analysis of shape and volume changes in activated thrombocytes in real time by single-shot spatial light modulator-based differential interference contrast imaging
✍ Scribed by Saranjam Khan; Alexander Jesacher; Walter Nussbaumer; Stefan Bernet; Monika Ritsch-Marte
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 537 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1864-063X
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✦ Synopsis
Abstract
We suggest to use a combination of optical tweezers and single‐image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially designed phase mask displayed at the SLM enables quantitative phase calculation from only a single recording. The optical tweezers stabilize trapped thrombocytes for long‐time monitoring of changes in the optical thickness profile of thrombocytes during activation by adenosine diphosphate (ADP). (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)