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Quantitative analysis of cell adhesion on aligned micro- and nanofibers

✍ Scribed by Furong Tian; Hossein Hosseinkhani; Mohsen Hosseinkhani; Ali Khademhosseini; Yoshiro Yokoyama; Giovani Gomez Estrada; Hisatoshi Kobayashi

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
328 KB
Volume
84A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

In this study, we quantitatively analyzed the affinity of cell adhesion to aligned nanofibers composed of composites of poly(glycolic acid) (PGA) and collagen. Electrospun composite fibers were fabricated at various PGA/collagen weight mixing ratio (7, 18, 40, 67, and 86%) to generate fibers that ranged in diameter from 10 ΞΌm to 500 nm. Scanning electron microscopy (SEM) observation revealed that the PGA/collagen fibers were long and uniformly aligned, irrespective of the PGA/collagen weight mixing ratio. In addition, it was observed that a significantly higher number of NIH3T3 fibroblasts adhered to nanofibers with smaller diameters in comparison to fibers with larger diameters. The highest affinity of cell adhesion was observed in the PGA/collagen fibers with diameter of 500 nm and PGA/collagen weight mixing ratio of 40%. Furthermore, the adherent cells were more elongated on fibers with smaller diameters. Thus, based on the results here, PGA/collagen composite fibers are suitable for tissue culture studies and provide an attractive material for tissue engineering applications. Β© 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

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