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Quantitative analysis of caffeic acid phenethyl ester in crude propolis by liquid chromatography-electrospray ionization mass spectrometry

✍ Scribed by Piero Del Boccio; Domenico Rotilio

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
124 KB
Volume
27
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antinflammatory, and immunomodulatory properties. The paper describes a rapid and simple liquid chromatography‐electrospray ionisation mass spectrometry method for qualitative and quantitative determination of CAPE. The chromatographic separation was performed with a Luna RP‐C~18~ column using a water‐acetonitrile linear gradient. The method was linear over a 0.125–80 ng/mL range (LOD = 62.5 pg/mL). The method was applied for the quantitation of caffeic acid phenethyl ester in crude propolis samples, which were analysed directly after extraction with ethyl acetate solution.

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