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Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry

✍ Scribed by D.L. Hachey; S.P. Coburn; L.T. Brown; W.F. Erbelding; B. DeMark; P.D. Klein

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
775 KB
Volume
151
Category
Article
ISSN
0003-2697

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✦ Synopsis

Methods have been developed for the simultaneous quantitative analysis of vitamin B6 forms in biological samples by isotope dilution mass spectrometry using deuterated forms of pyridoxine, pyridoxal, pyridoxamine, and pyridoxic acid. The biological fluid or tissue sample was homogenized and then treated with a cocktail containing appropriate amounts of each deuterated vitamer, as well as the deuterated, phosphorylated vitamer forms. The individual vitamers were isolated from the homogenate by a complex high-performance liquid chromatographic procedure that provided separate fractions for each of the six vitamers found in biological samples. Aldehydic B6 vitamers were reduced to the alcohol form prior to acetylation and analysis by gas chromatography/mass spectrometry (GC/MS). The three resulting vitamers were analyzed by electron ionization GC/ MS using a silicone capillary column. The methods have been applied to analysis of vitamin B6 in liver, milk, urine, and feces at levels as low as 0.02 nmol/ml. o 1985 Academic PITS, hc.

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