Quantitation of urapidil and its metabolites in human serum by high performance liquid chromatography

✍ Scribed by Nieder, M. ;Dilger, C. ;Haerlin, R.

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 382 KB
• Volume: 8
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240080503

✦ Synopsis

The antihypertensive agent urapidil (Ebrantil Byk-Gulden, Konstanz) can be reliably quantitated with three metabolites in human serum using high performance liquid chromatography. Serum is alkalinized and extracted with ethyl acetate. The organic phase is back-extracted with diluted acid. An aliquot is sampled automatically and chromatographed in an optimized combination of mobile and stationary phase. UV-detection at 273 nm allows a quantitation limit of 5 ng/ml for all analytes.

Precise handling of exact volumes facilitates external calibration. The coefficient of variation for spiked samples is less than 5% within and less than 7% between studies. Application of the method to experimental and clinical pharmacokinetic studies of urapidil is illustrated.