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Quantitation of rice glutelin, an acid/alkali/urea soluble protein, by enzyme linked immunosorbent assay

✍ Scribed by Y. R. Mawal; M. R. Mawal; M. N. Sainani; P. K. Ranjekar

Book ID
104647717
Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
240 KB
Volume
4
Category
Article
ISSN
0951-208X

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✦ Synopsis

A sensitive and rapid ELISA for quantitation of rice glutelin was developed. This method employed binding of rice glutelin to Immulon I microtitre plate and quantitating it with commercial goat anti-rabbit IgG conjugate by conventional ELISA. Using this procedure, glutelin as low as 5ng could be detected.

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