A simple method for the estimation of specific mRNA concentrations in small tissue samples (as little as 1 mg) or cultured cells (lower limit 10(5) cells) is described. Guanidine hydrochloride extracts of whole cells or tissues are applied directly onto nitrocellulose and hybridized with the appropr
Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization
✍ Scribed by H. Yamamoto; K. Nose; N. Itoh; H. Okamoto
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 232 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1420-682X
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