Quantitation of non-ideal behavior in protein size-exclusion chromatography
β Scribed by Paul L. Dubin; Shun L. Edwards; Mamta S. Mehta; Donald Tomalia
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 200 KB
- Volume
- 635
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The mechanisms governing the broadening of experimental chromatograms for proteins and paucidisperse dextrans were studied on TSK-G2000SW and TSK-G3000SW columns. Within the conditions studied, the chromatogram variance for all solutes increased linearly with increasing effluent flow rate. As predic
Size exclusion chromatography (SEC) is the most widely used method for aggregation analysis of pharmaceutical proteins. However SEC analysis has a number of limitations, and one of the most important ones is protein adsorption to the resin. This problem is particularly severe when using new columns,
## e z -C a m i n o and G. M a r q u e z -R u i z " In this paper, separation of the polar compounds previously obtained from heated fats by column chromatography is proposed in order to increase knowledge of the alteration of frying fats. The analysis is carried out in 35 min by high performance s