Quantitation of individual proteins by a two-stage electrophoresis procedure

✍ Scribed by Alanna Ruddell; Marcelo Jacobs-Lorena

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 370 KB
• Volume: 122
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90276-7

✦ Synopsis

A technique has been developed for quantitating specific proteins present in a complex mixture, using a two-stage electrophoresis procedure. It consists of homogenizing a relevant portion of an isoelectric focusing gel into a slot of sodium dodecyl sulfate-polyacrylamide slab gel. After electrophoresis, proteins form bands which can be stained and analyzed by standard densitometric procedures. Radioactively labeled marker protein is used to monitor recovery, so that accurate quantitative measurements can be obtained. This method has been used to measure the abundance of actin in Drosophila egg chambers. The technique is applicable to the quantitation of many specific proteins among complex protein populations.