2.5 0.5 1.96 { 0.06 1.08 2.5 1.0 1.47 { 0.04 1.03 2.5 1.5 0.97 { 0.03 1.02 4.0 1.0 2.96 { 0.06 1.04 4.0 2.0 1.96 { 0.05 1.02 Use of Tris(2-carboxyethyl)phosphine for 4.0 3.0 0.96 { 0.03 1.
Quantitation of Hydrogen Peroxide Using Tris(2-carboxyethyl)phosphine
โ Scribed by Joan Han; Shau Yen; Grace Han; Peter Han
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 65 KB
- Volume
- 234
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A simple spectrophotometric method for quantitative determination of hypochlorite (OCl 0 ) or hypochlorous acid (HOCl) is described. The OCl 0 or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as t
The concentration of tris(2-carboxyethyl)phosphine (TCEP) can be conveniently determined by measuring the amount of 2-nitro-5-thiobenzoate (NTB) formed after reaction with \(5,5^{\prime}\)-dithiobis(2-nitrobenzoic acid) (DTNB). This method utilizes the fact that TCEP reduces DTNB rapidly and stoichi
The newly introduced sulfhydryl reductant tris(2-carboxyethyl)phosphine (TCEP) is a potentially attractive alternative to commonly used dithiothreitol (DTT). We compare properties of DTT and TCEP important in protein biochemistry, using the motor enzyme myosin as an example protein. The reductants e