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Quantitation of Hydrogen Peroxide Using Tris(2-carboxyethyl)phosphine

โœ Scribed by Joan Han; Shau Yen; Grace Han; Peter Han


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
65 KB
Volume
234
Category
Article
ISSN
0003-2697

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2.5 0.5 1.96 { 0.06 1.08 2.5 1.0 1.47 { 0.04 1.03 2.5 1.5 0.97 { 0.03 1.02 4.0 1.0 2.96 { 0.06 1.04 4.0 2.0 1.96 { 0.05 1.02 Use of Tris(2-carboxyethyl)phosphine for 4.0 3.0 0.96 { 0.03 1.

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A simple spectrophotometric method for quantitative determination of hypochlorite (OCl 0 ) or hypochlorous acid (HOCl) is described. The OCl 0 or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as t

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The newly introduced sulfhydryl reductant tris(2-carboxyethyl)phosphine (TCEP) is a potentially attractive alternative to commonly used dithiothreitol (DTT). We compare properties of DTT and TCEP important in protein biochemistry, using the motor enzyme myosin as an example protein. The reductants e