Quantitation of human cytomegalovirus DNA in peripheral blood leukocytes of heart transplant recipients: relationship with pp65 antigenemia and with antiviral therapy
β Scribed by Valeria Ghisetti; Anna Barbui; Fabrizia Pittaluga; Enrico Donegani; Marco Bobbio; Michele di Summa; Giovanna Marchiaro
- Book ID
- 111107855
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 785 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1198-743X
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The use of sensitive and specific methods for rapid and reliable diagnosis is required due to the considerable impact of human cytomegalovirus (HCMV) in organ transplant recipients. For this purpose the demonstration of the presence of viral antigens in peripheral blood leukocytes (PMNLs) and of vir
## Abstract After bone marrow (BM) or solidβorgan (SO) transplantation viremic __Cytomegalovirus__ (CMV) infection is observed frequently. Quantitative assay of CMV in blood helps the management of this clinical condition. In the present report, 83 samples from 39 solid organ recipients, three CMV
The polymerase chain reaction (PCR) for cytomegalovirus (CMV) DNA quantitation provides sensitive and specific data for detecting CMV as well as monitoring the infection and determining the appropriate antiviral strategy. A recently introduced real-time PCR assay for CMV DNA quantitation was applied