Quantitation of ethidium-stained closed circular DNA in agarose gels
โ Scribed by Michael F Shubsda; Jerry Goodisman; James C Dabrowiak
- Book ID
- 117331438
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 327 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0165-022X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
There are excellent methods available for separating superhelical DNA (covalently closed rings) from a mixture of open circular DNA (rings with one or more single-strand interruptions) and linear DNA (1,2). The separation of open circular DNA from linear DNA presents a more difficult problem. While
We have developed a method for the comparison of quantitative differences between nucleic acid samples run on agarose gels. It is useful for the analysis of digested genomic DNA and RNA preparations, as well as for clamped homogeneous electric field gel analysis of damaged chromosomal DNA. The techn