Quantitation of dose-dependent increases in CYP1A1 messenger RNA levels in juvenile chinook salmon exposed to treated bleached-kraft mill effluent using two field sampling techniques
✍ Scribed by Pamela M. Campbell; George M. Kruzynski; Ian K. Birtwell; Robert H. Devlin
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 103 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0730-7268
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Increases in hepatic CYP1A1 messenger RNA (mRNA) levels were quantitated, using a reverse‐transcriptase–competitive‐polymerase‐chain‐reaction assay, from juvenile chinook salmon following exposure to treated bleached‐kraft mill effluent (BKME). Two tissue preservation techniques, alcohol fixation and freezing, were compared and both found to be valid, although absolute values obtained differed between the methods. The results of this study show that juvenile chinook respond with a dose‐dependent induction of CYP1A1 mRNA to levels of treated BKME found under winter, low‐flow conditions downstream of pulp mills in the Fraser River, British Columbia, Canada. The validation of alcohol fixation as a sampling technique for studies on mRNA levels improves the ease of field sampling and increases the feasibility of using CYP1A1 mRNA level as a bioindicator of exposure of fish to specific chemical contaminants.