QUANTITATION OF DNA DAMAGE IN NON-RADIOACTIVE DNA
โ Scribed by Betsy M. Sutherland; Giovanni Ciarrocchi; Marina Ciomei; John C. Sutherland
- Book ID
- 114894827
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 975 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0031-8655
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A sensitive assay for quantitating DNA damage within individual genes would be a valuable tool for identifying the molecular mechanisms of disease and the sites of action of various carcinogens and anticancer drugs. This report describes a competitive PCR assay that was used to quantitate DNA damage
A batch elution procedure is described for quantitative measurement of DNA damage. The technique is based on alkaline unwinding of cellular DNA and separation of singlestranded from duplex forms by step elution from hydroxylapatite with phosphate formamide. The method is rapid, permits large numbers