Quantitation of diltiazem and desacetyldiltiazem in dog plasma by high-performance liquid chromatography

✍ Scribed by Robert E. Wiens; Dennis J. Runser; Joseph P. Lacz; Dan C. Dimmitt

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 234 KB
• Volume: 73
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600730525

✦ Synopsis

0 A high-performance liquid chromatographic procedure was developed for the determination of diltiazem and desacetyldiltiazem in dog plasma. Two milliliters of plasma is extracted with a hexane-2-propanol mixture. The assay uses a reverse-phase column maintained at 55°C with a silica saturation column and a pellicular precolumn. The mobile phase is acetonitrile-water (5050) at pH 6.6 with 1 S-g/L heptanesulfonic acid added as the ion-pair reagent. The procedure is sensitive to 5 ng/mL for both compounds in dog plasma and is linear up to 2000 ng/mL for diltiazem and lo00 ng/mL for desacetyldiltiazem. Preliminary dog mean plasma profiles of diltiazem and desacetyldiltiazem are presented.

Keyphrases 0 Diltiazem-quantitation with desacetyldiltiazem, dog plasma, HPLC 0 Desacetyldiltiazem-quantitation with diltiazem, dog plasma, HPLC 0 HPLC-quantitation of diltiazern and desacetyldiltiazem, dog plasma