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Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays

✍ Scribed by Martin Oppermann; Horst Baumgarten; Elke Brandt; Walter Gottsleben; Christian Kurts; Otto Götze

Book ID
118902016
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
798 KB
Volume
133
Category
Article
ISSN
0022-1759

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An enzyme-linked immunosorbent assay (ELISA) has been adapted to measure E. coli recA protein in the 1 to 10 ng range in whole-cell sonicates, membrane extracts, and osmotic shock fluid from 2 x 10(8) cells. The specific activity of recA protein is maintained at a relatively constant "basal' level (