Quantitation of antigen in radial immunodiffusion plates
โ Scribed by Peter Schmid
- Book ID
- 115818399
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 118 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0009-8981
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๐ SIMILAR VOLUMES
An economical alternative to the determination of proteins by single radial diffusion in antibody-containing agarose gel is described. The method, which is the equivalent ofthe above, consists of allowing the sample to diffuse into the gel layer, overlaying of the sample well with antibody-impregnat
Using known principles of radial immunodiffusion it is shown experimentally that an heterologous antigen may be quantified using an homologous antigen-antibody system. The proteins in which this is demonstrated are human serum albumin (the homologous antigen) and the plasma albumins of baboon (Papio