✦ LIBER ✦

Quantitation and detection of isotypes of ANTI-SS-B antibodies by elisa and farr assays using affinity purified antigens

✍ Scribed by PATRICK J. W. Venables; Peter J. Charles; RUSSELL R. C. Buchanan; Tung Yi; Patricia A. Mumford; Leslie Schrieber; GERALDINE R. W. Room; Ravinder N. Maini

Publisher: John Wiley and Sons
Year: 1983
Tongue: English
Weight: 804 KB
Volume: 26
Category: Article
ISSN: 0004-3591
DOI: 10.1002/art.1780260205

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✦ Synopsis

Abstract

Affinity purified SS‐B was characterized as a protein with immunoreactive polypeptides of 40K and 29K. A modified Farr assay and an enzyme linked immunosorbent assay (ELISA) were 100‐ to 1,000‐fold more sensitive than immunodiffusion and showed an association with the systemic manifestations of primary sicca syndrome and Sjögren's syndrome with systemic lupus erythematosus. The ELISA was sufficiently sensitive to detect class specific antibodies in saliva and lymphocyte culture supernatants.

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