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Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

✍ Scribed by Wang, Weixun; Zhou, Haihong; Lin, Hua; Roy, Sushmita; Shaler, Thomas A.; Hill, Lander R.; Norton, Scott; Kumar, Praveen; Anderle, Markus; Becker, Christopher H.


Book ID
120059202
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
216 KB
Volume
75
Category
Article
ISSN
0003-2700

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A quantitative GLC-mass spectrometric assay was developed for the determination of cocaine and its principal metabolite, benzoylecgonine, in human urine. The assay utilizes selective ion focusing to monitor in a GLC effluent the molecular ions of cocaine and benzoylecgonine generated by electron-imp