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Quantification of Phosphatidylinositol 3,4,5-Trisphosphate by Liposome Lysis Assay with Specific Monoclonal Antibodies

โœ Scribed by Yuichiro Nishio; Satoshi Nagata; Masato Umeda; Ryuichi Shirai; Tohhei Yokogawa; Sayoko Ihara; Yasuhisa Fukui


Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
64 KB
Volume
285
Category
Article
ISSN
0003-2697

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A new method has been developed to monitor glycosyltransferase and glycosylhydrolase activities. Reaction product identification and quantification are accomplished simultaneously with an enzyme-linked immunosorbent assay (ELISA) using carbohydrate sequence-specific monoclonal antibodies. P-Galactos