Quantification of osteopontin in human plasma with an ELISA: Basal levels in pre- and postmenopausal women

✍ Scribed by Diosdado S. Bautista; Zahida Saad; Ann F. Chambers; Katia S. Tonkin; Frances P. O'Malley; Hemant Singhal; Sonya Tokmakejian; Vivien Bramwell; John F. Harris

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 834 KB
• Volume: 29
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(96)84728-a

✦ Synopsis

Objectives:

To develop an immunoassay for osteopontin (opn), a secreted phosphoglycoprotein that is implicated in a number of human diseases, and establish basal plasma opn levels in healthy women.

Design and methods:

An antigen-capture elisa was developed to quantity opn in plasma using a combination of mouse monoclonal and rabbit polyclonal antibodies. basal opn levels were determined in blood plasma of 21 pre- and 14 postmenopausal women obtained at 7-day intervals over a 4-week period.

Results:

A group of 35 healthy women had a median opn level of 31 micrograms/l (range = 14-64 micrograms/l). comparison between pre- and postmenopausal women showed that their 4-week average opn levels did not differ significantly (p > 0.16, mann-whitney test), and that levels in each premenopausal individual remained constant during the menstrual cycle, unaffected by cyclical levels of leuteinizing hormone and progesterone.

Conclusion:

Systematic quantification of plasma opn can now be done by elisa, which was used to establish basal plasma opn levels in a group of healthy women. levels in pre- and postmenopausal women appeared relatively stable over a 4-week period.