Quantification of Lipids, Liposomes, Amino Acids, and Proteins by Thermal Ultramicrodigestion and an Ultramicrocoulometric Assay, Based on the Reaction of Hypobromite with Ammonia

✍ Scribed by S. Zellmer; G. Kaltenborn; U. Rothe; H. Lehnich; J. Lasch; H.-D. Pauer

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 59 KB
• Volume: 273
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1999.4214

✦ Synopsis

The quantification of nitrogen in organic material is described. It is based on a novel thermal ultramicrodigestion in combination with an ultramicrocoulometric quantification. The lower detection limit of the coulometric measurement is 0.5 g nitrogen, which corresponds to 20 g lipid, 3 g glycine, or 4 g protein. Therefore it is as sensitive as the frequently used Lowry method. In contrast to the Lowry protein determination it is not disturbed by detergents and most other interfering substances.