𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantification of Individual Oligosaccharide Compounds from Human Milk Using High-pH Anion-Exchange Chromatography

✍ Scribed by Stephan Thurl; Beate Müller-Werner; Günther Sawatzki

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
93 KB
Volume
235
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A quantification method suitable for determination of individual oligosaccharide compounds from human milk has been established. The crude milk oligosaccharide fraction was separated into acidic oligosaccharides, neutral oligosaccharides, and lactose by gel permeation chromatography. After this separation step neutral and acidic oligosaccharides were analyzed separately by high-pH anion-exchange chromatography with pulsed amperometric detection. The concentrations of 14 neutral oligosaccharides, of 6 acidic oligosaccharides, and of N-acetylneuraminic acid were determined on the internal standards stachyose and galacturonic acid, respectively. Thus, previously applied quantification methods for milk oligosaccharides based on gel permeation chromatography have been decisively improved.

📜 SIMILAR VOLUMES

High-performance anion exchange-chromato
High-performance anion exchange-chromatography of neutral milk oligosaccharides and oligosaccharide alditols derived from mucin glycoproteins
✍ G.P. Reddy; C. Allen Bush 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 659 KB

High-performance anion-exchange (HPAE) chromatography under alkaline conditions (pH approximately 13) has been used to separate neutral oligosaccharides from human milk as well as oligosaccharide alditols isolated by alkaline borohydride degradation of O-linked glycoproteins having blood group A and

Separation of fucosylated oligosaccharid
Separation of fucosylated oligosaccharides using high-pH anion-exchange chromatography with pulsed-amperometric detection
✍ Mark R. Hardy; R.Reid Townsend 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 450 KB

Fucosylated oligosaccharides of the /3Gal(l+4)GlcNAc-, PGal(l+4)Glc-, and /3Gal(l+3)GlcNAc+eries were chromatographed on a high-performance anion-exchange pellicular resin under alkaline conditions (pH ~13). Fucosylation of either lactose, lactosamine (Type II chains), or lacto-N-biose (Type I chain