This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive
Quantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinoma
β Scribed by Danny Ka-Ho Wong; Man-Fung Yuen; Ronnie Tung-Ping Poon; John Chi-Hang Yuen; James Fung; Ching-Lung Lai
- Book ID
- 116651137
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 303 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0168-8278
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## Abstract Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is responsible for viral persistence. This study aimed to investigate the serum surrogate markers for cccDNA and to evaluate the intrahepatic viral events associated with disease activity in HBeAgβnegative chronic hepatitis
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## Abstract Epigenetic factors may modulate chronic Hepatitis B viral infection by affecting virion gene transcription. The aim of this study was to compare the methylation status of the intrahepatic covalently closed circular DNA (cccDNA) CpG island 2 and HBV replication capability. HBV cccDNA was