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Quantification of gene expression of Listeria monocytogenes by real-time reverse transcription PCR: Optimization, evaluation and pitfalls

✍ Scribed by Hadewig Werbrouck; Nadine Botteldoorn; Mieke Uyttendaele; Lieve Herman; Els Van Coillie


Book ID
116750273
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
289 KB
Volume
69
Category
Article
ISSN
0167-7012

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## Abstract The solid tumor mRNA expression of genes related to the mechanism of action of certain antineoplastic agents is often predictive of clinical efficacy. We report here on the development of a rapid and practical real‐time RT‐PCR method to quantify genetic expression in solid tumors. The g