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Quantification of CBFB-MYH11 fusion gene levels in paired peripheral blood and bone marrow samples by real-time PCR

✍ Scribed by Boeckx, N; De Roover, J; van der Velden, V H J; Maertens, J; Uyttebroeck, A; Vandenberghe, P; van Dongen, J J M


Book ID
110056482
Publisher
Nature Publishing Group
Year
2005
Tongue
English
Weight
114 KB
Volume
19
Category
Article
ISSN
0887-6924

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Detection and quantification of CBFB/MYH
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We used a newly established real-time RT-PCR assay for the quantification of the leukemia-specific CBFB/MYH11 transcripts in inv( 16)-positive acute myeloblastic leukemia. CBFB/MYH11 could be quantified over a five log range, with a detection limit of 10 molecules of a CBFB/MYH11 plasmid and a 1:10