Quantification of Bacterial Polysaccharides by the Purpald Assay: Measurement of Periodate-Generated Formaldehyde from Glycol in the Repeating Unit

Quantification of Bacterial Lipopolysacc

Quantification of Bacterial Lipopolysaccharides by the Purpald Assay: Measuring Formaldehyde Generated from 2-keto-3-deoxyoctonate and Heptose at the Inner Core by Periodate Oxidation

✍ Che-Hung Lee; Chao-Ming Tsai 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 88 KB

We have adapted the purpald assay (M. S. Quesenberry and Y. C. Lee, Anal. Biochem. 234, 50-55, 1996) to quantify lipopolysaccharide (LPS) content in solution in 96-well microtiter plates at room temperature. This method employs the oxidation of unsubstituted terminal vicinal glycol groups in 2-keto-