Quantification of 32P-labeled samples in gel fragments using the flat-bed liquid scintillation counter

✍ Scribed by C.G. Potter; C.C. Tan; P.J. Ratcliffe

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 955 KB
• Volume: 197
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90366-2

✦ Synopsis

Quantification of 32P in bands after gel electrophoresis was performed using the flat-bed scintillation counter (Betaplate). The most convenient system involved placing fragments of dried gel between two glass fiber sheets, each previously sealed in a thin plastic bag with liquid scintillant. Good pulse-height spectra and counting efficiencies were obtained with low cross talk and background. The method has been used to quantify mRNA in RNA antisense-protection assays that were linear over a wide range (1-20000 cpm). Cross talk and background could be reduced further by an alternative technique utilizing plastic trays with shallow wells in which a solid scintillant had been melted. Fragments were immersed in the molten scintillant (90 degrees C), which was allowed to solidify, by cooling, before counting.