Quality of Original and Biosimilar Epoetin Products

✍ Scribed by Vera Brinks; Andrea Hawe; Abdul H. H. Basmeleh; Liliana Joachin-Rodriguez; Rob Haselberg; Govert W. Somsen; Wim Jiskoot; Huub Schellekens

• Book ID: 106494098
• Publisher: Springer US
• Year: 2010
• Tongue: English
• Weight: 332 KB
• Volume: 28
• Category: Article
• ISSN: 0724-8741
• DOI: 10.1007/s11095-010-0288-2

✦ Synopsis

Purpose

To compare the quality of therapeutic erythropoietin (EPO) products, including two biosimilars, with respect to content, aggregation, isoform profile and potency.

Methods

Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency.

Results

Tested EPO products differed in content, isoform composition, and potency.

Conclusion

Of the tested products, the biosimilars have the same or even better quality as the originals. Especially, the potency of originals may significantly differ from the value on the label.