Qualitative PCR for the detection of chicken and pork adulteration in goose and mule duck foie gras
✍ Scribed by Miguel A Rodríguez; Teresa García; Isabel González; Luis Asensio; Pablo E Hernández; Rosario Martin
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 240 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Polymerase chain reaction amplification of a conserved region of the α‐actin gene has been used for specific identification of chicken (Gallus gallus) and pork (Sus scrofa domesticus) adulteration in goose (Anser anser) and mule duck (Anasplatyrhynchos × Cairinamoschata) foie gras. The design of species‐specific forward primers, together with a reverse universal primer, allowed the generation of amplicons of different lengths in each species. The different sizes of the species‐specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of the presence chicken and pork in goose and mule duck foie gras with a detection limit of 0.1% (w/w). The technique could be used in inspection programmes to enforce labelling regulation of foie gras and other meat products. Copyright © 2003 Society of Chemical Industry