Qualitative and quantitative changes in β-1,4-glucosidase accompanying growth of Aspergillus nidulans

✍ Scribed by S. Sharma; P. S. Bagga; Dr. D. K. Sandhu

• Book ID: 102393953
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 402 KB
• Volume: 30
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620300513

✦ Synopsis

Abstract

A comparative study of the wild type A. nidulans and a mutant strain aco‐T69 (lacking conidia and cleistothecia) revealed the better production of β‐1,4‐glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also showed a variation. Highest specific activity was found in the cleistothecial extracts. The electrophoretic analysis of the wild type strain demonstrated the presence of three isoenzymes of β‐1,4‐glucosidase i.e. β‐GLU I, β‐GLU II and β‐GLU III, the number of which varies from one to three during the growth of the organism. The parallel study of the mutant strain showed complete absence of β‐GLU II. All three forms were present in the somatic hyphae and cleistothecial extracts while spore extracts depicted the absence of β‐GLU III in the wild type.