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Pyruvate Formate-Lyase of Escherichia coli: the Acetyl-Enzyme Intermediate

✍ Scribed by Joachim KNAPPE; Hans P. BLASCHKOWSKI; Peter GRÖBNER; Thomas SCHMTTT

Book ID
115114470
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
868 KB
Volume
50
Category
Article
ISSN
1432-1327

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A mutant of Escherichia coli deficient i
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A genetic study was performed on a mutation which has caused loss of pyruvate formate lyase. The gene affected is designated pfl and is located close to ser C, i.e. at 20 mn on the chromosomal map of E. coli.

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## Abstract The product of __yfi__D gene is similar to pyruvate formate‐lyase (PFL) activase and it has been reported to activate PFL by replacing the glycyl radical domain. To quantitate the effect of YfiD on the cell metabolism in microaerobic cultures, glucose‐limited chemostat cultures were con