Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA

C-Terminal Modifications of a Protein by

C-Terminal Modifications of a Protein by UAG-Encoded Incorporation of Puromycin during in vitro Protein Synthesis in the Absence of Release Factor 1

✍ Dmitry E. Agafonov; Kersten S. Rabe; Michael Grote; C. Stefan Voertler; Mathias 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 285 KB

## Abstract __Deactivation of release factor 1 by polyclonal antibodies in an in vitro translation system, which was used to express the esterase gene, led to the reversible elimination of naturally occurring termination. This technique allowed the antibiotic puromycin to be used as an acceptor sub