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Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping

✍ Scribed by Royo, Jose Luis; Hidalgo, Manuel; Ruiz, Agustin

Book ID
109946220
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
263 KB
Volume
2
Category
Article
ISSN
1750-2799

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## Abstract Mutations in the __KRAS__ gene are very important diagnostic and prognostic markers in cancer. Particularly, __KRAS__ mutations at codons 12 and 13 have a high prognostic value for EGFR‐directed antibody therapies. Several methods are available to detect the most common mutations, some