Pyrophosphate contamination in crystalline phosphocreatine: A source of error in kinetic studies using coupled enzymes

✍ Scribed by Carl Moos; I-Nan M. Feng

• Book ID: 102626183
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 358 KB
• Volume: 87
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90595-x

✦ Synopsis

Commercial crystalline phosphocreatine generally contains a contaminant, probably pyrophosphate, that can inhibit nucleoside triphosphatases and may therefore cause errors in steady-state kinetic studies of creatine kinasecoupled reactions. Ion-exchange chromatography reveals the presence of pyrophosphate in some batches of phosphocreatine sufficient to account for the observed inhibition. Chromatographically purified phosphocreatine shows no inhibitory effect.