Pyrolysis/gas chromatography/mass spectrometry of spelt (Triticum spelta L.) pericarp

✍ Scribed by Guido C. Galletti; L. Filippo D’Antuono; Paola Bocchini; Isabella Rosolen

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 81 KB
• Volume: 12
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19981130)12:22<1801::aid-rcm375>3.0.co;2-i

✦ Synopsis

Pyrolysis/gas chromatography/mass spectrometry (PY/GC/MS) was used to characterize the pericarp fiber of grain samples of 6 spelt (Triticum spelta L.) along with 3 different bread wheats plus 7 spelt-wheat crosses. Absolute amounts of lignin and polysaccaride pyrolysis products were quantified as mg mg À1 of sample using an internal standard method. The pyrolysis fragments ascribed to the polysaccharide fraction were significantly more abundant in spelt whereas wheats contained the highest percentage of ligninderived pyrolysis fragments. Discriminant analysis differentiated spelt, spelt-wheat crosses and wheat. Thus, among possible options for fiber analysis, PY/GC/MS proved to be a reproducible and reliable analytical tool, which can be easily applied to the screening and characterization of cereal fiber.