Pyrimidine 5'-nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography

✍ Scribed by Chiara Zanone; Laurent R. Chiarelli; Giovanna Valentini; Eleonora Perani; Laura Annovazzi; Simona Viglio; Paolo Iadarola

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 111 KB
• Volume: 25
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200406057

✦ Synopsis

Abstract

A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'‐nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'‐azido‐3'‐deoxythymidine as the phosphate acceptor.