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Pyrene derivatives as fluorescent probes of conformation near the 3′ termini of polyribonucleotides

✍ Scribed by Paul Koenig; Scott A. Reines; Charles R. Cantor

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
588 KB
Volume
16
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

When pyrene butyric acid hydrazide or pyrene acetic acid hydrazide is attached to single‐strand RNA 3′ termini a red shift in absorbance and substantial hypochromicity are observed. A strong induced CD is seen and the fluorescence intensity is quenched by an order of magnitude. In double‐stranded samples, a further 10‐fold quenching of fluorescence is seen. Several lines of evidence suggest that the residual fluorescence of pyrene butyric acid hydrazide‐duplex conjugates arises from a minor species. The most likely possibility is dye reacted at a site other than the 3′ end. Some indication exists that 3′‐attached pyrene may perturb the relative stability of nearby duplex. Within the limits of this reservation, it appears that 3′‐pyrene conjugates may be rather useful for detecting the existence of duplex regions accessible to a dye at the 3′ end of complex RNAs.

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Excimer formation of pyrene as a probe to investigate the recombination of geminate pairs: ODESR and fluorescence study of dilute pyrene in squalane
✍ Masaharu Okazaki; Yutaka Tai; Ryoichi Nakagaki; Keichi Nunome; Kazumi Toriyama 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 504 KB

It is shown that the monomer fluorescence and the excimer fluorescence from a dilute pyrene solution in squalane at room temperature are mainly from "delayed" geminate pairs (Py-, S+) and (Py-, Py+ ), respectively, from the ODESR spectra observed at the monomer and the excimer fluorescence. The reco