Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles

Acridine orange as a probe for measuring

Acridine orange as a probe for measuring pH gradients across membranes: Mechanism and limitations

✍ Michael Gjedde Palmgren 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 694 KB

Acridine orange is an optical probe commonly used to monitor pH gradients across membranes. In the present study, the changes observed in the visible absorption spectrum of acridine orange during intravesicular acidification of oat root plasma membrane vesicles are shown to be identical with those o