Puromycin enhances 20-hydroxyecdysone-induced protein synthesis in wing discs ofDrosophila melanogaster

✍ Scribed by Frank Y. T. Sin

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 341 KB
• Volume: 12
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00368874

✦ Synopsis

The effect of cycloheximide and puromycin on 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster has been studied by one-dimensional and two-dimensional SDS polyacrylamide electrophoresis. It is found that puromycin, but not cycloheximide, when applied simultaneously with the hormone enhanced the hormone-induced synthesis of the 'early' and 'late' proteins. However, when puromycin was applied after hormone treatment, only the 'late' proteins were induced. The possible implication of these observations is discussed.