Purinoceptor-stimulated phosphoinositide hydrolysis in Madin-Darby canine kidney (MDCK) cells
β Scribed by C.-M. Yang; Yih-Jeng Tsai; Shiow-Lin Pan; Chuen-Tao Tsai; Wen-Bin Wu; Chi-Tso Chiu; Shu-Fen Luo; Jonathan T. Ou
- Book ID
- 105743553
- Publisher
- Springer-Verlag
- Year
- 1997
- Tongue
- English
- Weight
- 120 KB
- Volume
- 356
- Category
- Article
- ISSN
- 0028-1298
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According to previous studies hyposmotic swelling of Madin Darby Canine Kidney (MDCK) cells leads to a marked decrease of cell membrane resistance. The present study has been performed to identify the underlying ion channels using the patch-clamp technique: reduction of extracellular osmolarity to 2
## Abstract Cation transport systems were investigated in mutant MadinβDarby Canine Kidney (MDCK) cells resistant to the diuretic drug amiloride. The mutants were isolated previously as clones resistant to the cytotoxic effects of 3 Γ 10^β4^ M amiloride. Decreased amiloride transport by the Na^+^ c