Purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced byBordetella pertussisandBordetella bronchiseptica
✍ Scribed by Timothy J. Brickman; Jan-Gerd Hansel; Marvin J. Miller; Sandra K. Armstrong
- Book ID
- 104640825
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 827 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1572-8773
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✦ Synopsis
Hydroxamate siderophores of virulent Bordetella pertussis and Bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(S),11,18(S)- tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone+ ++, which was previously isolated from the taxonomically-related bacterial species Alcaligenes denitrificans subsp. xylosoxydans. Alcaligin purified from iron-depleted cultures of B. pertussis and B. bronchiseptica exhibited specific growth-promoting activity under iron-restricted conditions for Bordetella indicator strains, and ere active in [55Fe]ferric alcaligin transport assays. Evidence suggests that several C2-symmetric conformations of alcaligin exist simultaneously in both methanolic and aqueous solution.