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Purification, Properties, and Phosphorylation by Protein Kinase C of Two Phosphoinositidase C Isozymes from Rat Brain

✍ Scribed by Jonathan L. Blank; Keith A. Foster; John N. Hawthorne


Book ID
111176035
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
852 KB
Volume
57
Category
Article
ISSN
0022-3042

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A calcium-sensitive, phospholipid-dependent protein kinase (protein kinase C) and its three isozymes were purified from rat heart cytosolic fractions utilizing a rapid purification method. The purified protein kinase C enzyme showed a single polypeptide band of 80 KDa on SDS-polyacrylamide gel elect